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61.
Megumi Kuroiwa Keisuke Koba Kazuo Isobe Ryunosuke Tateno Asami Nakanishi Yoshiyuki Inagaki Hiroto Toda Shigeto Otsuka Keishi Senoo Yuichi Suwa Muneoki Yoh Rieko Urakawa Hideaki Shibata 《Journal of Forest Research》2011,16(5):363-373
Measurements of gross NH 4 + and NO 3 ? production in forest soils were conducted using the 15N pool dilution method. Mineral topsoils (0?C10?cm depth) were collected from four forests from northern to southern Japan with a natural climate gradient to elucidate the mechanisms regulating gross nitrification rates in forest soils. Additionally, we attempted to evaluate the relative importance of heterotrophic nitrification in gross total nitrification using acetylene as a specific inhibitor of autotrophic nitrification. Distinct differences were found among sites in the gross rates of NH 4 + production (3.1?C11.4?mg?N?kg?1?day?1) and gross total nitrification (0.0?C6.1?mg?N?kg?1?day?1). The rates of gross heterotrophic nitrification were low in this study, indicating that heterotrophic nitrification is of minor importance in most forest mineral topsoils in Japan. Significant relations were found between gross autotrophic nitrification and gross NH 4 + production, soil N, and soil C concentrations, but none was found between gross autotrophic nitrification and soil pH. We determined the critical value of the gross NH 4 + production rates for gross autotrophic nitrification under which no gross autotrophic nitrification occurred, as well as the critical soil C/N ratio above which gross autotrophic nitrification ceased. Results show that tight coupling of production and consumption of NH 4 + prevents autotrophic nitrifiers from utilizing NH 4 + as long as NH 4 + availability is low. 相似文献
62.
Haruo Shindo Daisuke Watanabe Tatsuya Onaga Makiko Urakawa Osamu Nakahara Qiaoyun Huang 《Soil Science and Plant Nutrition》2013,59(5):763-767
Abstrac The effects of 3 oxides (Fe, Al, and Mn oxides) and 3 clay minerals (kaolin, montmorillonite, and allophane) on the adsorption and subsequent kinetic properties of acid phosphatase were compared. The amount of enzyme adsorbed by the oxides and clay minerals followed the order: montmorillonite ? kaolin > Mn oxide > Fe oxide > Al oxide ? allophane. The adsorption isotherms of the enzyme on the oxides and clay minerals, except for montmorillonite and allophane, fitted the Langmuir equation. The activity of the enzyme immobilized by the inorganic components studied was in the order of allophane > kaolin > Fe oxide > montmorillonite > Al oxide ≒ Mn oxide. Compared to the free enzyme, the V max, Km, and V max / K m values of the immobilized enzyme decreased, increased, and decreased, respectively. Among the oxides or clay minerals, the higher the ability of the inorganic components to adsorb the enzyme, the lower the value of the V max / K m ratio of the immobilized enzyme. These findings suggest that the catalytic efficiency of the enzyme complexes formed is determined by the adsorbability of the inorganic components for the enzyme. 相似文献
63.
Megumi Fukuzawa Naomi Hayashi 《Journal of Veterinary Behavior: Clinical Applications and Research》2013,8(4):221-224
Effective dog training involves reinforcement of the dog's correct actions in response to specific cues given by the trainer. Food is usually selected as the primary reinforcer (reward), although this selection does not necessarily account for the learning preferences of individual dogs. We evaluated the relationship between reward and learning efficiency. Fifteen dogs were allocated to 3 different reward groups (food, stroking, and praise) and trained by an identical process. The food reward was the only one that shortened the time taken for the response to the command to be completed. However, this difference occurred only in the early training stages and not later in the training process. 相似文献
64.
Megumi YOSHIDA Keijiro MIZUKAMI Masaharu HISASUE Ichiro IMANISHI Keigo KURATA Masaki OCHIAI Masato ITOH Tadahiro NASUKAWA Jumpei UCHIYAMA Hajime TSUJIMOTO Masahiro SAKAGUCHI 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(8):1202
Severe adverse reactions after rabies vaccination in dogs were examined from 317 cases reported to the Ministry of Agriculture, Forestry and Fisheries (MAFF) in Japan during 15-year period from April 2004 to March 2019. We found that 109 of the 317 dogs showed anaphylaxis (0.15/100,000 vaccinated dogs), and 71 of the 109 cases of anaphylaxis resulted in death (0.10/100,000 vaccinated dogs). We measured bovine serum albumin (BSA) in four commercially available rabies vaccines and found the levels ranged from 0.1 to 16.6 µg/dose. Our survey showed that the rate of anaphylaxis to rabies vaccines in dogs is rare, although some cases of anaphylaxis resulted in death. Veterinarians should be well prepared to deal with vaccine-associated anaphylaxis. 相似文献
65.
Asami Yoshida Megumi Ohta Koichi Kuwahara Min-Jie Cao Kenji Hara Kiyoshi Osatomi 《Marine drugs》2015,13(11):6550-6565
An endogenous protease in fish muscle, cathepsin B, was partially purified and characterized from horse mackerel meat. On SDS-PAGE of the purified enzyme under reducing conditions, main protein bands were detected at 28 and 6 kDa and their respective N-terminal sequences showed high homology to heavy and light chains of cathepsin B from other species. This suggested that horse mackerel cathepsin B formed two-chain forms, similar to mammalian cathepsin Bs. Optimum pH and temperature of the enzyme were 5.0 and 50 °C, respectively. A partial cDNA encoding the amino acid sequence of 215 residues for horse mackerel cathepsin B was obtained by RT-PCR and cloned. The deduced amino acid sequence contains a part of light and heavy chains of cathepsin B. The active sites and an N-glycosylation site were conserved across species. We also confirmed that the modori phenomenon was avoided by CA-074, a specific inhibitor for cathepsin B. Therefore, our results suggest that natural cysteine protease inhibitor(s), such as oryzacystatin derived from rice, can apply to thermal-gel processing of horse mackerel to avoid the modori phenomenon. Meanwhile, this endogenous protease may be used for food processing, such as weaning meal and food for the elderly. 相似文献
66.
Nobuya Tashiro Ayako Urakawa Kazuyuki Shoji Yoichi Matsuo Toyozo Sato 《Journal of General Plant Pathology》2018,84(5):381-385
In early January 2015–2017, anthracnose was detected on Satsuma mandarin orange (SMO) (Citrus unshiu) fruits kept in farmers’ storage rooms in Saga Prefecture, Japan. Three single-spore isolates from rotten fruits reproduced the postharvest anthracnose symptoms in wound-inoculated SMO fruits and were re-isolated from lesions. The isolates were identified as Colletotrichum fioriniae based on conidial morphology, culture characteristics, rDNA-ITS, and beta-tubulin-2 gene sequences. This is the first report of postharvest anthracnose on SMO caused by C. fioriniae. 相似文献
67.
Arai T Inoue A Takeguchi A Mizutani H Shimoo M Sako T Yoshimura I Kimura N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(11):1241-1243
Concentrations of plasma glucose, immunoreactive insulin (IRI) and free fatty acid (FFA) and activities of enzymes related to energy metabolism and lactate dehydrogenase (LDH) isoenzyme pattern in plasma and leukocytes were investigated in lactating Holstein cows (dairy cattle) and fattening Japanese Black Wagyu x Holstein steers (beef cattle). IRI concentrations and LDH and malate dehydrogenase (MDH) activities in the plasma of beef cattle were significantly higher than those in dairy cattle. The cytosolic ratio of MDH/LDH activity in the leukocytes of beef cattle was significantly higher than that of dairy cattle. These findings might be associated with the different energy metabolism between dairy and beef cattle. 相似文献
68.
Gamoh K Shimazaki Y Makie H Senda M Itoh O Inoue Y 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):1027-1029
Canine parvovirus type-2a (CPV-2a) and type-2b (CPV-2b) have recently been isolated from domestic cats. The pathogenicity of CPV-2b in domestic cats is still unclear. In this study, we performed infection tests to examine the pathogenicity of CPV-2b, FP84 strain, isolated from a domestic cat. The results demonstrated that the CPV strain FP84 is able to infect and replicate well in domestic cats. Two of the 3 cats used in the test died. They showed loss of appetite, diarrhea, leukopenia and dehydration. Since FP84 was found to be virulent to domestic cats, it is necessary to examine the efficacy of inactivated feline panleukopenia virus vaccines against CPV infection in domestic cats. 相似文献
69.
70.
Masaru Nakano Toshikazu Nomizu Keiko Mizunashi Mami Suzuki Shiro Mori Sachiko Kuwayama Megumi Hayashi Hiroto Umehara Eriko Oka Hiroyuki Kobayashi Megumi Asano Shintaro Sugawara Hiroki Takagi Hiroyuki Saito Masashi Nakata Toshinari Godo Yoshihiro Hara Junji Amano 《Scientia Horticulturae》2006,110(4):366-371
The Liliaceous perennial Tricyrtis hirta, sometimes called ‘Japanese toad lily’, has recently become popular as an ornamental for pot and garden uses. Highly embryogenic callus cultures of this plant predominately consisted of diploid cells but also contained tetraploid cells after 1 year of establishment. In the present study, plans regenerated from the 1-year-old embryogenic callus cultures were subjected to ploidy level analysis and morphological characterization following 3 years of cultivation. Among 37 plants examined, 28 kept the diploid level (2n = 2x = 26) but nine were tetraploid (2n = 4x = 52) as indicated by FCM analysis and chromosome observation. Although no morphological alterations were detected in 26 out of 28 diploid regenerants, the remaining two showed noticeable variations: both were severely dwarf and had crimped leaves and many malformed flowers. The tetraploid regenerants had several horticulturally attractive characteristics compared with the diploid controls, such as longer shoots, thicker stems, and larger flowers. Thus regeneration of tetraploid plants from 1-year-old embryogenic callus cultures offers a possibility to improve the horticultural value of T. hirta, although regeneration of trueness-to-type plants is essential for utilizing the cultures for micropropagation and genetic transformation. 相似文献